Functional variability of the Lr34 durable resistance gene in transgenic wheat
Identifieur interne : 000262 ( Main/Exploration ); précédent : 000261; suivant : 000263Functional variability of the Lr34 durable resistance gene in transgenic wheat
Auteurs : Joanna M. Risk [Australie] ; Liselotte L. Selter [Suisse] ; Simon G. Krattinger [Australie, Suisse] ; Libby A. Viccars [Australie] ; Terese M. Richardson [Australie] ; Gabriele Buesing [Suisse] ; Gerhard Herren [Suisse] ; Evans S. Lagudah [Australie] ; Beat Keller [Suisse, Australie]Source :
- Plant Biotechnology Journal [ 1467-7644 ] ; 2012-05.
English descriptors
- KwdEn :
- Teeft :
- Adult plant resistance, Adult plants, Adult stage, Appl, Authors plant biotechnology journal, Biologist, Biotechnology, Blackwell publishing, Callose, Callose deposition, Cell death, Chlorophyll catabolites, Chlorotic, Cold treatment, Colony area, Colony size, Cultivar, Durable resistance, Dyck, Endogenous, Experimental biology, Expression levels, Fungal, Fungal pathogens, Gene, Gene expression, Genet, Genetic background, Glasshouse, Hypersensitive response, Infection, Infection sites, Kolmer, Krattinger, Lagudah, Leaf, Leaf rust, Leaf rust resistance, Leaf rust resistance gene, Leaf samples, Mackellar, Microscopic analysis, Mildew, Negative controls, Pathogen, Pathogen infection, Pathol, Phenotype, Plant biotechnology journal, Plant microbe, Plant pathol, Powdery mildew, Primer, Qpcr, Quantitative resistance, Rust, Second leaf, Seedling, Seedling resistance, Seedling stage, Singh, Standard glasshouse conditions, Statistical analysis, Stripe rust, Thatcher, Theor, Transcript, Transcript levels, Transcription factors, Transgene, Transgenic, Transgenic lines, Transgenic wheat, Transgenic wheat lines, Trend lines, Wheat.
Abstract
Breeding for durable disease resistance is challenging, yet essential to improve crops for sustainable agriculture. The wheat Lr34 gene is one of the few cloned, durable resistance genes in plants. It encodes an ATP binding cassette transporter and has been a source of resistance against biotrophic pathogens, such as leaf rust (Puccinina triticina), for over 100 years. As endogenous Lr34 confers quantitative resistance, we wanted to determine the effects of transgenic Lr34 with specific reference to how expression levels affect resistance. Transgenic Lr34 wheat lines were made in two different, susceptible genetic backgrounds. We found that the introduction of the Lr34 resistance allele was sufficient to provide comparable levels of leaf rust resistance as the endogenous Lr34 gene. As with the endogenous gene, we observed resistance in seedlings after cold treatment and in flag leaves of adult plants, as well as Lr34‐associated leaf tip necrosis. The transgene‐based Lr34 resistance did not involve a hypersensitive response, altered callose deposition or up‐regulation of PR genes. Higher expression levels compared to endogenous Lr34 were observed in the transgenic lines both at seedling as well as adult stage and some improvement of resistance was seen in the flag leaf. Interestingly, in one genetic background the transgenic Lr34‐based resistance resulted in improved seedling resistance without cold treatment. These data indicate that functional variability in Lr34‐based resistance can be created using a transgenic approach.
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DOI: 10.1111/j.1467-7652.2012.00683.x
Affiliations:
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The wheat Lr34 gene is one of the few cloned, durable resistance genes in plants. It encodes an ATP binding cassette transporter and has been a source of resistance against biotrophic pathogens, such as leaf rust (Puccinina triticina), for over 100 years. As endogenous Lr34 confers quantitative resistance, we wanted to determine the effects of transgenic Lr34 with specific reference to how expression levels affect resistance. Transgenic Lr34 wheat lines were made in two different, susceptible genetic backgrounds. We found that the introduction of the Lr34 resistance allele was sufficient to provide comparable levels of leaf rust resistance as the endogenous Lr34 gene. As with the endogenous gene, we observed resistance in seedlings after cold treatment and in flag leaves of adult plants, as well as Lr34‐associated leaf tip necrosis. The transgene‐based Lr34 resistance did not involve a hypersensitive response, altered callose deposition or up‐regulation of PR genes. Higher expression levels compared to endogenous Lr34 were observed in the transgenic lines both at seedling as well as adult stage and some improvement of resistance was seen in the flag leaf. Interestingly, in one genetic background the transgenic Lr34‐based resistance resulted in improved seedling resistance without cold treatment. These data indicate that functional variability in Lr34‐based resistance can be created using a transgenic approach.</div></front></TEI><affiliations><list><country><li>Australie</li><li>Suisse</li></country><region><li>Canton de Zurich</li></region><settlement><li>Zurich</li></settlement><orgName><li>Université de Zurich</li></orgName></list><tree><country name="Australie"><noRegion><name sortKey="Risk, Joanna M" sort="Risk, Joanna M" uniqKey="Risk J" first="Joanna M." last="Risk">Joanna M. Risk</name></noRegion><name sortKey="Keller, Beat" sort="Keller, Beat" uniqKey="Keller B" first="Beat" last="Keller">Beat Keller</name><name sortKey="Krattinger, Simon G" sort="Krattinger, Simon G" uniqKey="Krattinger S" first="Simon G." last="Krattinger">Simon G. Krattinger</name><name sortKey="Lagudah, Evans S" sort="Lagudah, Evans S" uniqKey="Lagudah E" first="Evans S." last="Lagudah">Evans S. Lagudah</name><name sortKey="Lagudah, Evans S" sort="Lagudah, Evans S" uniqKey="Lagudah E" first="Evans S." last="Lagudah">Evans S. Lagudah</name><name sortKey="Richardson, Terese M" sort="Richardson, Terese M" uniqKey="Richardson T" first="Terese M." last="Richardson">Terese M. Richardson</name><name sortKey="Viccars, Libby A" sort="Viccars, Libby A" uniqKey="Viccars L" first="Libby A." last="Viccars">Libby A. Viccars</name></country><country name="Suisse"><region name="Canton de Zurich"><name sortKey="Selter, Liselotte L" sort="Selter, Liselotte L" uniqKey="Selter L" first="Liselotte L." last="Selter">Liselotte L. Selter</name></region><name sortKey="Buesing, Gabriele" sort="Buesing, Gabriele" uniqKey="Buesing G" first="Gabriele" last="Buesing">Gabriele Buesing</name><name sortKey="Herren, Gerhard" sort="Herren, Gerhard" uniqKey="Herren G" first="Gerhard" last="Herren">Gerhard Herren</name><name sortKey="Keller, Beat" sort="Keller, Beat" uniqKey="Keller B" first="Beat" last="Keller">Beat Keller</name><name sortKey="Krattinger, Simon G" sort="Krattinger, Simon G" uniqKey="Krattinger S" first="Simon G." last="Krattinger">Simon G. Krattinger</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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